Valine 181 is critical for the nucleotide exchange activity of human mitochondrial ADP/ATP carriers in yeast

Carine De Marcos Lousa, Véronique Trézéguet (Lead / Corresponding author), Claudine David, Vincent Postis, Bertrand Arnou, Eva Pebay-Peyroula, Gérard Brandolin, Guy J.M. Lauquin

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

We isolated yeast Saccharomyces cerevisiae cells transformed with one of the three human adenine nucleotide carrier genes (HANC) that exhibited higher growth capacity than previously observed. The HANC genes were isolated from these clones, and we identified two independent mutations of HANC that led to replacement of valine 181 located in the fourth transmembrane segment by methionine or phenylalanine. Tolerance of this position toward substitution with various amino acids was systematically investigated, and since HANC/V181M was among the most efficient in growth complementation, it was more extensively studied. Here we show that increased growth capacities were associated with higher ADP/ATP exchange activities and not with higher human carrier amount in yeast mitochondria. These results are discussed in the light of the bovine Ancp structure, that shares more than 90% amino acid identity with Hancps, and its interaction with the lipid environment.

Original languageEnglish
Pages (from-to)4342-4348
Number of pages7
JournalBiochemistry
Volume44
Issue number11
DOIs
Publication statusPublished - 22 Mar 2005

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