VAMPIRE® fundus image analysis algorithms: Validation and diagnostic relevance in hypertensive cats

Alessandro Cirla; Michele Drigo; Lucia Ballerini; Emanuele Trucco; Giovanni Barsotti

doi:10.1111/vop.12657

VAMPIRE® fundus image analysis algorithms: Validation and diagnostic relevance in hypertensive cats

Alessandro Cirla (Lead / Corresponding author)
, Michele Drigo
, Lucia Ballerini
, Emanuele Trucco
, Giovanni Barsotti

Computing

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

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Abstract

Objectives: To validate a retinal imaging software named VAMPIRE® (Vascular Assay and Measurement Platform for Images of the Retina) in feline patients and test the clinical utility in hypertensive cats.

Animals Studied: One hundred and five healthy cats were enrolled. They represented the normal dataset used in the validation (group 1). Forty-three hypertensive cats with no noticeable retinal abnormalities were enrolled for the clinical validity of the software (group 2).

Procedures: Eleven points (4 veins, 4 arteries, and 3 arterial bifurcations) were measured for each digital image. Repeatability and reproducibility of measurements were assessed using two independent operators. Data were statistically analyzed by the Mann-Whiney and Tukey box plot. Significance was considered when P < 0.05.

Results: Two hundred and ten retinal images were analyzed for a total of 2310 measurements. Total mean was 9.1 and 6.1 pixels for veins and arteries, respectively. First, second, and third arteriolar bifurcations angles were 73.6°, 76.9°, and 85.4°, respectively. A comparison between groups 1 and 2 showed a statistically significant reduction in arteriolar diameter (mean 3.3 pixels) and branch angle (55°, 47.8° and 59.9°) associated with increasing vein diameter (mean 24.15 pixels).

Conclusions: Current image analysis techniques used in human medicine were investigated in terms of extending their use to veterinary medicine. The VAMPIRE® algorithm proved useful for an objective diagnosis of retinal vasculature changes secondary to systemic hypertension in cats, and could be an additional diagnostic test for feline systemic hypertension.

Original language	English
Pages (from-to)	819-827
Number of pages	9
Journal	Veterinary Ophthalmology
Volume	22
Issue number	6
Early online date	22 Feb 2019
DOIs	https://doi.org/10.1111/vop.12657
Publication status	Published - 1 Nov 2019

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

cat
fundus
image analysis algorithms
retinal photography
software validation
systemic hypertension

ASJC Scopus subject areas

General Veterinary

Access to Document

10.1111/vop.12657

Author Accepted ManuscriptAccepted author manuscript, 1.32 MB

Cite this

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title = "VAMPIRE{\textregistered} fundus image analysis algorithms: Validation and diagnostic relevance in hypertensive cats",

abstract = "Objectives: To validate a retinal imaging software named VAMPIRE{\textregistered} (Vascular Assay and Measurement Platform for Images of the Retina) in feline patients and test the clinical utility in hypertensive cats.Animals Studied: One hundred and five healthy cats were enrolled. They represented the normal dataset used in the validation (group 1). Forty-three hypertensive cats with no noticeable retinal abnormalities were enrolled for the clinical validity of the software (group 2).Procedures: Eleven points (4 veins, 4 arteries, and 3 arterial bifurcations) were measured for each digital image. Repeatability and reproducibility of measurements were assessed using two independent operators. Data were statistically analyzed by the Mann-Whiney and Tukey box plot. Significance was considered when P < 0.05.Results: Two hundred and ten retinal images were analyzed for a total of 2310 measurements. Total mean was 9.1 and 6.1 pixels for veins and arteries, respectively. First, second, and third arteriolar bifurcations angles were 73.6°, 76.9°, and 85.4°, respectively. A comparison between groups 1 and 2 showed a statistically significant reduction in arteriolar diameter (mean 3.3 pixels) and branch angle (55°, 47.8° and 59.9°) associated with increasing vein diameter (mean 24.15 pixels).Conclusions: Current image analysis techniques used in human medicine were investigated in terms of extending their use to veterinary medicine. The VAMPIRE{\textregistered} algorithm proved useful for an objective diagnosis of retinal vasculature changes secondary to systemic hypertension in cats, and could be an additional diagnostic test for feline systemic hypertension.",

keywords = "cat, fundus, image analysis algorithms, retinal photography, software validation, systemic hypertension",

author = "Alessandro Cirla and Michele Drigo and Lucia Ballerini and Emanuele Trucco and Giovanni Barsotti",

note = "{\textcopyright} 2019 American College of Veterinary Ophthalmologists.",

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T2 - Validation and diagnostic relevance in hypertensive cats

AU - Cirla, Alessandro

AU - Drigo, Michele

AU - Ballerini, Lucia

AU - Trucco, Emanuele

AU - Barsotti, Giovanni

PY - 2019/11/1

Y1 - 2019/11/1

N2 - Objectives: To validate a retinal imaging software named VAMPIRE® (Vascular Assay and Measurement Platform for Images of the Retina) in feline patients and test the clinical utility in hypertensive cats.Animals Studied: One hundred and five healthy cats were enrolled. They represented the normal dataset used in the validation (group 1). Forty-three hypertensive cats with no noticeable retinal abnormalities were enrolled for the clinical validity of the software (group 2).Procedures: Eleven points (4 veins, 4 arteries, and 3 arterial bifurcations) were measured for each digital image. Repeatability and reproducibility of measurements were assessed using two independent operators. Data were statistically analyzed by the Mann-Whiney and Tukey box plot. Significance was considered when P < 0.05.Results: Two hundred and ten retinal images were analyzed for a total of 2310 measurements. Total mean was 9.1 and 6.1 pixels for veins and arteries, respectively. First, second, and third arteriolar bifurcations angles were 73.6°, 76.9°, and 85.4°, respectively. A comparison between groups 1 and 2 showed a statistically significant reduction in arteriolar diameter (mean 3.3 pixels) and branch angle (55°, 47.8° and 59.9°) associated with increasing vein diameter (mean 24.15 pixels).Conclusions: Current image analysis techniques used in human medicine were investigated in terms of extending their use to veterinary medicine. The VAMPIRE® algorithm proved useful for an objective diagnosis of retinal vasculature changes secondary to systemic hypertension in cats, and could be an additional diagnostic test for feline systemic hypertension.

AB - Objectives: To validate a retinal imaging software named VAMPIRE® (Vascular Assay and Measurement Platform for Images of the Retina) in feline patients and test the clinical utility in hypertensive cats.Animals Studied: One hundred and five healthy cats were enrolled. They represented the normal dataset used in the validation (group 1). Forty-three hypertensive cats with no noticeable retinal abnormalities were enrolled for the clinical validity of the software (group 2).Procedures: Eleven points (4 veins, 4 arteries, and 3 arterial bifurcations) were measured for each digital image. Repeatability and reproducibility of measurements were assessed using two independent operators. Data were statistically analyzed by the Mann-Whiney and Tukey box plot. Significance was considered when P < 0.05.Results: Two hundred and ten retinal images were analyzed for a total of 2310 measurements. Total mean was 9.1 and 6.1 pixels for veins and arteries, respectively. First, second, and third arteriolar bifurcations angles were 73.6°, 76.9°, and 85.4°, respectively. A comparison between groups 1 and 2 showed a statistically significant reduction in arteriolar diameter (mean 3.3 pixels) and branch angle (55°, 47.8° and 59.9°) associated with increasing vein diameter (mean 24.15 pixels).Conclusions: Current image analysis techniques used in human medicine were investigated in terms of extending their use to veterinary medicine. The VAMPIRE® algorithm proved useful for an objective diagnosis of retinal vasculature changes secondary to systemic hypertension in cats, and could be an additional diagnostic test for feline systemic hypertension.

KW - cat

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KW - image analysis algorithms

KW - retinal photography

KW - software validation

KW - systemic hypertension

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JO - Veterinary Ophthalmology

JF - Veterinary Ophthalmology

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ER -

VAMPIRE® fundus image analysis algorithms: Validation and diagnostic relevance in hypertensive cats

Abstract

UN SDGs

Keywords

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