Variant Analyses of Candidate Genes in Orofacial Clefts in Multi-Ethnic Populations

Mary Li, Joy Olotu, Carmen J. Buxo-Martinez, Peter A. Mossey, Deepti Anand, Tamara Busch, Azeez Alade, Lord J. J. Gowans, Mekonen Eshete, Wasiu L. Adeyemo, Thirona Naicker, Waheed O. Awotoye, Sagar Gupta, Chinyere Adeleke, Valeria Bravo, Siyong Huang, Olatunbosun O. Adamson, Ada M. Toraño, Carolina A. Bello, Mairim SotoMarilyn Soto, Ricardo Ledesma, Myrellis Marquez, Jose F. Cordero, Lydia M. Lopez-Del Valle, Maria I. Salcedo, Natalio Debs, Aline Petrin, Hannah Malloy, Khalid Elhadi, Olutayo James, Mobolanle O. Ogunlewe, Fekir Abate, Abiye Hailu, Ibrahim Mohammed, Paul Gravem, Milliard Deribew, Mulualem Gesses, Mohaned Hassan, John Pape, Solomon Obiri-Yeboah, Fareed K. N. Arthur, Alexander A. Oti, Peter Donkor, Mary L. Marazita, Salil A. Lachke, Adebowale A. Adeyemo, Jeffrey C. Murray, Azeez Butali (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
109 Downloads (Pure)


Objectives: Cleft lip with/without cleft palate and cleft palate only are congenital birth defects where the upper lip and/or palate fail to fuse properly during embryonic facial development. Affecting ~1.2/1000 live births world-wide, these orofacial clefts impose significant social and financial burdens on affected individuals and their families. Orofacial clefts have a complex etiology resulting from genetic variants combined with environmental covariates. Recent genome-wide association studies and whole-exome sequencing for orofacial clefts identified significant genetic associations and variants in several genes. Of these, we investigated the role of common/rare variants in SHH, RORA, MRPL53, ACVR1, and GDF11.

Materials and methods: We sequenced these five genes in 1255 multi-ethnic cleft lip with/without palate and cleft palate only samples in order to find variants that may provide potential explanations for the missing heritability of orofacial clefts. Rare and novel variants were further analyzed using in-silico predictive tools.

Results: 19 total variants of interest were found, with variant types including stop-gain, missense, synonymous, intronic, and splice-site variants. Of these, 3 novel missense variants were found, one in SHH, one in RORA, and one in GDF11.

Conclusion: This study provides evidence that variants in SHH, RORA, MRPL53, ACVR1, and GDF11 may contribute to risk of orofacial clefts in various populations.

Original languageEnglish
Pages (from-to)1921-1935
Number of pages15
JournalOral Diseases
Issue number7
Early online date1 Jun 2021
Publication statusPublished - Oct 2022


  • candidate gene
  • congenital birth defect
  • craniofacial genetics
  • genome-wide association studies
  • novel variants
  • whole-exome sequencing

ASJC Scopus subject areas

  • Otorhinolaryngology
  • General Dentistry


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