Vascular endothelial growth factor in patients with critical limb ischemia before and after amputation

M. McLaren, D. J. Newton, F. Khan, J. J. F. Belch

    Research output: Contribution to journalArticle

    11 Citations (Scopus)

    Abstract

    Background. It is known that levels of vascular endothelial growth factor (VEGF) in biological fluids increase with inflammation and vascular proliferation. Theraputic angiogenesis by injection of VEGF or genes encoding for it may be a promising strategy for treatment of critical limb ischemia. However growth factors are also implicated in the development of vascular disease by smooth muscle cell proliferation. Methods. We have previously shown VEGF levels to be increased in juvenile diabetic subjects with no clinical evidence of vascular disease. We have measured serum VEGF using an enzyme linked immunosorbent assay and cellular VEGF expression using flow cytometry in patients with critical limb ischemia prior to and 6 months postamputation to determine whether removal of the ischemic limb leads to changes in systemic endothelial cell function. Results. Baseline VEGF levels were significantly increased in the patient group compared to controls with levels returning to control levels at 6 months postsurgery. Monocyte and neutrophil VEGF expression was significantly reduced in the patient group. Platelet expression of VEGF was also reduced but this failed to reach statistical significance. Conclusions. The results suggest that it may be useful to determine the balance between VEGF production and cellular receptor expression prior to treatment.
    Original languageEnglish
    Pages (from-to)165-168
    Number of pages4
    JournalInternational Angiology
    Volume21
    Issue number2
    Publication statusPublished - 2002

    Fingerprint

    Amputation
    Vascular Endothelial Growth Factor A
    Ischemia
    Extremities
    Vascular Diseases
    Vascular Smooth Muscle
    Smooth Muscle Myocytes
    Blood Vessels
    Monocytes
    Intercellular Signaling Peptides and Proteins
    Flow Cytometry
    Neutrophils
    Blood Platelets
    Endothelial Cells
    Enzyme-Linked Immunosorbent Assay
    Cell Proliferation
    Inflammation
    Injections
    Therapeutics
    Serum

    Cite this

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    title = "Vascular endothelial growth factor in patients with critical limb ischemia before and after amputation",
    abstract = "Background. It is known that levels of vascular endothelial growth factor (VEGF) in biological fluids increase with inflammation and vascular proliferation. Theraputic angiogenesis by injection of VEGF or genes encoding for it may be a promising strategy for treatment of critical limb ischemia. However growth factors are also implicated in the development of vascular disease by smooth muscle cell proliferation. Methods. We have previously shown VEGF levels to be increased in juvenile diabetic subjects with no clinical evidence of vascular disease. We have measured serum VEGF using an enzyme linked immunosorbent assay and cellular VEGF expression using flow cytometry in patients with critical limb ischemia prior to and 6 months postamputation to determine whether removal of the ischemic limb leads to changes in systemic endothelial cell function. Results. Baseline VEGF levels were significantly increased in the patient group compared to controls with levels returning to control levels at 6 months postsurgery. Monocyte and neutrophil VEGF expression was significantly reduced in the patient group. Platelet expression of VEGF was also reduced but this failed to reach statistical significance. Conclusions. The results suggest that it may be useful to determine the balance between VEGF production and cellular receptor expression prior to treatment.",
    author = "M. McLaren and Newton, {D. J.} and F. Khan and Belch, {J. J. F.}",
    year = "2002",
    language = "English",
    volume = "21",
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    journal = "International Angiology",
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    }

    Vascular endothelial growth factor in patients with critical limb ischemia before and after amputation. / McLaren, M.; Newton, D. J.; Khan, F.; Belch, J. J. F.

    In: International Angiology, Vol. 21, No. 2, 2002, p. 165-168.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Vascular endothelial growth factor in patients with critical limb ischemia before and after amputation

    AU - McLaren, M.

    AU - Newton, D. J.

    AU - Khan, F.

    AU - Belch, J. J. F.

    PY - 2002

    Y1 - 2002

    N2 - Background. It is known that levels of vascular endothelial growth factor (VEGF) in biological fluids increase with inflammation and vascular proliferation. Theraputic angiogenesis by injection of VEGF or genes encoding for it may be a promising strategy for treatment of critical limb ischemia. However growth factors are also implicated in the development of vascular disease by smooth muscle cell proliferation. Methods. We have previously shown VEGF levels to be increased in juvenile diabetic subjects with no clinical evidence of vascular disease. We have measured serum VEGF using an enzyme linked immunosorbent assay and cellular VEGF expression using flow cytometry in patients with critical limb ischemia prior to and 6 months postamputation to determine whether removal of the ischemic limb leads to changes in systemic endothelial cell function. Results. Baseline VEGF levels were significantly increased in the patient group compared to controls with levels returning to control levels at 6 months postsurgery. Monocyte and neutrophil VEGF expression was significantly reduced in the patient group. Platelet expression of VEGF was also reduced but this failed to reach statistical significance. Conclusions. The results suggest that it may be useful to determine the balance between VEGF production and cellular receptor expression prior to treatment.

    AB - Background. It is known that levels of vascular endothelial growth factor (VEGF) in biological fluids increase with inflammation and vascular proliferation. Theraputic angiogenesis by injection of VEGF or genes encoding for it may be a promising strategy for treatment of critical limb ischemia. However growth factors are also implicated in the development of vascular disease by smooth muscle cell proliferation. Methods. We have previously shown VEGF levels to be increased in juvenile diabetic subjects with no clinical evidence of vascular disease. We have measured serum VEGF using an enzyme linked immunosorbent assay and cellular VEGF expression using flow cytometry in patients with critical limb ischemia prior to and 6 months postamputation to determine whether removal of the ischemic limb leads to changes in systemic endothelial cell function. Results. Baseline VEGF levels were significantly increased in the patient group compared to controls with levels returning to control levels at 6 months postsurgery. Monocyte and neutrophil VEGF expression was significantly reduced in the patient group. Platelet expression of VEGF was also reduced but this failed to reach statistical significance. Conclusions. The results suggest that it may be useful to determine the balance between VEGF production and cellular receptor expression prior to treatment.

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    EP - 168

    JO - International Angiology

    JF - International Angiology

    SN - 0392-9590

    IS - 2

    ER -