Vasoactive intestinal peptide (VIP) induces a K+ current in the Xenopus oocyte membrane

Vincenzina Reale; Michael L. J. Ashford; Eric A. Barnard

doi:10.1016/0304-3940(87)90221-7

Vasoactive intestinal peptide (VIP) induces a K+ current in the Xenopus oocyte membrane

Vincenzina Reale, Michael L. J. Ashford, Eric A. Barnard

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Evidence, based on voltage-clamp and current-clamp recording, is presented for endogenous receptors for vasoactive intestinal polypeptide (VIP) on the cell membrane of the Xenopus oocyte. At normal resting potential a hyperpolarisation is produced by VIP, associated with an increase in K+ conductance. The response is dose-dependent, with a threshold near 10(-9)M VIP. The effect is potentiated by forskolin. Of several other types of neuropeptide tested, only avian pancreatic polypeptide gives a response: this is similar to that of VIP, but this peptide can also potentiate the response to VIP.

Original language	English
Pages (from-to)	89-94
Number of pages	6
Journal	Neuroscience Letters
Volume	83
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(87)90221-7
Publication status	Published - 1987

Keywords

Xenopus laevis
Animals
Vasoactive Intestinal Peptide
Enzyme Activation
Ion Channels
Cell Membrane
Potassium
Oocytes
Membrane Potentials
Adenylate Cyclase
Forskolin

Access to Document

10.1016/0304-3940(87)90221-7

Cite this

@article{6c3c14caa5844ce2b56609b77a457b96,

title = "Vasoactive intestinal peptide (VIP) induces a K+ current in the Xenopus oocyte membrane",

abstract = "Evidence, based on voltage-clamp and current-clamp recording, is presented for endogenous receptors for vasoactive intestinal polypeptide (VIP) on the cell membrane of the Xenopus oocyte. At normal resting potential a hyperpolarisation is produced by VIP, associated with an increase in K+ conductance. The response is dose-dependent, with a threshold near 10(-9)M VIP. The effect is potentiated by forskolin. Of several other types of neuropeptide tested, only avian pancreatic polypeptide gives a response: this is similar to that of VIP, but this peptide can also potentiate the response to VIP.",

keywords = "Xenopus laevis, Animals, Vasoactive Intestinal Peptide, Enzyme Activation, Ion Channels, Cell Membrane, Potassium, Oocytes, Membrane Potentials, Adenylate Cyclase, Forskolin",

author = "Vincenzina Reale and Ashford, {Michael L. J.} and Barnard, {Eric A.}",

year = "1987",

doi = "10.1016/0304-3940(87)90221-7",

language = "English",

volume = "83",

pages = "89--94",

journal = "Neuroscience Letters",

issn = "0304-3940",

publisher = "Elsevier",

number = "1-2",

}

TY - JOUR

T1 - Vasoactive intestinal peptide (VIP) induces a K+ current in the Xenopus oocyte membrane

AU - Reale, Vincenzina

AU - Ashford, Michael L. J.

AU - Barnard, Eric A.

PY - 1987

Y1 - 1987

N2 - Evidence, based on voltage-clamp and current-clamp recording, is presented for endogenous receptors for vasoactive intestinal polypeptide (VIP) on the cell membrane of the Xenopus oocyte. At normal resting potential a hyperpolarisation is produced by VIP, associated with an increase in K+ conductance. The response is dose-dependent, with a threshold near 10(-9)M VIP. The effect is potentiated by forskolin. Of several other types of neuropeptide tested, only avian pancreatic polypeptide gives a response: this is similar to that of VIP, but this peptide can also potentiate the response to VIP.

AB - Evidence, based on voltage-clamp and current-clamp recording, is presented for endogenous receptors for vasoactive intestinal polypeptide (VIP) on the cell membrane of the Xenopus oocyte. At normal resting potential a hyperpolarisation is produced by VIP, associated with an increase in K+ conductance. The response is dose-dependent, with a threshold near 10(-9)M VIP. The effect is potentiated by forskolin. Of several other types of neuropeptide tested, only avian pancreatic polypeptide gives a response: this is similar to that of VIP, but this peptide can also potentiate the response to VIP.

KW - Xenopus laevis

KW - Animals

KW - Vasoactive Intestinal Peptide

KW - Enzyme Activation

KW - Ion Channels

KW - Cell Membrane

KW - Potassium

KW - Oocytes

KW - Membrane Potentials

KW - Adenylate Cyclase

KW - Forskolin

U2 - 10.1016/0304-3940(87)90221-7

DO - 10.1016/0304-3940(87)90221-7

M3 - Article

C2 - 2450310

SN - 0304-3940

VL - 83

SP - 89

EP - 94

JO - Neuroscience Letters

JF - Neuroscience Letters

IS - 1-2

ER -

Vasoactive intestinal peptide (VIP) induces a K+ current in the Xenopus oocyte membrane

Abstract

Keywords

Access to Document

Fingerprint

Cite this