Versatility of a localized surface plasmon resonance-based gold nanoparticle-alloyed quantum dot nanobiosensor for immunofluorescence detection of viruses

Kenshin Takemura, Oluwasesan Adegoke, Naoto Takahashi, Tatsuya Kato, Tian-Cheng Li, Noritoshi Kitamoto, Tomoyuki Tanaka, Tetsuro Suzuki, Enoch Y. Park (Lead / Corresponding author)

Research output: Contribution to journalArticlepeer-review

120 Citations (Scopus)


Flu infection, caused by the influenza virus, constitutes a serious threat to human lives worldwide. A rapid, sensitive and specific diagnosis is urgently needed for point-of-care treatment and to control the rapid spread of this disease. In this study, an ultrasensitive, rapid and specific localized surface plasmon resonance (LSPR)-induced immunofluorescence nanobiosensor has been developed for the influenza virus based on a gold nanoparticle (AuNP)-induced quantum dot (QD) fluorescence signal. Alloyed quaternary CdSeTeS QDs were synthesized via the hot-injection organometallic route and were subsequently capped with l-cysteine via a ligand exchange reaction. AuNPs were synthesized in HEPES buffer and thiolated with l-cysteine. The concept of the biosensor involves the conjugation of anti-neuraminidase (NA) antibody (anti-NA Ab) to thiolated AuNPs and the conjugation of anti-hemagglutinin (HA) antibody (anti-HA Ab) to alloyed quaternary l-cysteine-capped CdSeTeS QDs. Interaction of the antigens displaying on the surface of the influenza virus target with anti-NA Ab-conjugated AuNPs and anti-HA Ab-conjugated QDs induces an LSPR signal from adjacent AuNPs to trigger fluorescence-enhancement changes in the QDs in proportion to the concentration of the target virus. The detection limit for influenza H1N1 virus was 0.03pg/mL in deionized water and 0.4pg/mL in human serum; while, for the clinically isolated H3N2, the detection limit was 10PFU/mL. The detection of influenza virus H1N1 was accomplished with high sensitivity. The versatility of the biosensor was demonstrated for the detection of clinically isolated influenza virus H3N2 and norovirus-like particles (NoV-LPs).

Original languageEnglish
Pages (from-to)998-1005
Number of pages8
JournalBiosensors and Bioelectronics
Issue numberPt 2
Early online date20 Oct 2016
Publication statusPublished - 15 Mar 2017


  • Biosensing Techniques
  • Cysteine/chemistry
  • Gold/chemistry
  • Hemagglutinin Glycoproteins, Influenza Virus/isolation & purification
  • Humans
  • Influenza A Virus, H1N1 Subtype/chemistry
  • Influenza A Virus, H3N2 Subtype/chemistry
  • Influenza, Human/diagnosis
  • Limit of Detection
  • Metal Nanoparticles/chemistry
  • Quantum Dots/chemistry
  • Surface Plasmon Resonance


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