Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line

R. Z. Kozlowski, N. C. Sturgess, C. N. Hales, M. L. Ashford

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology.
    Original languageEnglish
    Pages (from-to)613-621
    Number of pages9
    JournalComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
    Volume100
    Issue number3
    DOIs
    Publication statusPublished - 1991

    Keywords

    • Rats
    • Animals
    • Membrane Potentials
    • Action Potentials
    • Islets of Langerhans
    • Models, Biological
    • Insulin
    • Cell Line

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