Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line

R. Z. Kozlowski; N. C. Sturgess; C. N. Hales; M. L. Ashford

doi:10.1016/0300-9629(91)90379-Q

Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line

R. Z. Kozlowski, N. C. Sturgess, C. N. Hales, M. L. Ashford

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology.

Original language	English
Pages (from-to)	613-621
Number of pages	9
Journal	Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
Volume	100
Issue number	3
DOIs	https://doi.org/10.1016/0300-9629(91)90379-Q
Publication status	Published - 1991

Keywords

Rats
Animals
Membrane Potentials
Action Potentials
Islets of Langerhans
Models, Biological
Insulin
Cell Line

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10.1016/0300-9629(91)90379-Q

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title = "Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line",

abstract = "1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology.",

keywords = "Rats, Animals, Membrane Potentials, Action Potentials, Islets of Langerhans, Models, Biological, Insulin, Cell Line",

author = "Kozlowski, {R. Z.} and Sturgess, {N. C.} and Hales, {C. N.} and Ashford, {M. L.}",

year = "1991",

doi = "10.1016/0300-9629(91)90379-Q",

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journal = "Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology",

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T1 - Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line

AU - Kozlowski, R. Z.

AU - Sturgess, N. C.

AU - Hales, C. N.

AU - Ashford, M. L.

PY - 1991

Y1 - 1991

N2 - 1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology.

AB - 1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology.

KW - Rats

KW - Animals

KW - Membrane Potentials

KW - Action Potentials

KW - Islets of Langerhans

KW - Models, Biological

KW - Insulin

KW - Cell Line

U2 - 10.1016/0300-9629(91)90379-Q

DO - 10.1016/0300-9629(91)90379-Q

M3 - Article

C2 - 1685973

SN - 1095-6433

VL - 100

SP - 613

EP - 621

JO - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

JF - Comparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology

IS - 3

ER -

Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line

Abstract

Keywords

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