Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line

R. Z. Kozlowski, N. C. Sturgess, C. N. Hales, M. L. Ashford

    Research output: Contribution to journalArticle

    3 Citations (Scopus)

    Abstract

    1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology.
    Original languageEnglish
    Pages (from-to)613-621
    Number of pages9
    JournalComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology
    Volume100
    Issue number3
    DOIs
    Publication statusPublished - 1991

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    Insulin-Secreting Cells
    Cell Line
    Electrophysiology
    Patch-Clamp Techniques
    Pharmacology

    Keywords

    • Rats
    • Animals
    • Membrane Potentials
    • Action Potentials
    • Islets of Langerhans
    • Models, Biological
    • Insulin
    • Cell Line

    Cite this

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    title = "Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line",
    abstract = "1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology.",
    keywords = "Rats, Animals, Membrane Potentials, Action Potentials, Islets of Langerhans, Models, Biological, Insulin, Cell Line",
    author = "Kozlowski, {R. Z.} and Sturgess, {N. C.} and Hales, {C. N.} and Ashford, {M. L.}",
    year = "1991",
    doi = "10.1016/0300-9629(91)90379-Q",
    language = "English",
    volume = "100",
    pages = "613--621",
    journal = "Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology",
    issn = "1095-6433",
    publisher = "Elsevier",
    number = "3",

    }

    Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line. / Kozlowski, R. Z.; Sturgess, N. C.; Hales, C. N.; Ashford, M. L.

    In: Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, Vol. 100, No. 3, 1991, p. 613-621.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - Voltage-activated currents in the CRI-G1 rat insulin-secreting cell-line

    AU - Kozlowski, R. Z.

    AU - Sturgess, N. C.

    AU - Hales, C. N.

    AU - Ashford, M. L.

    PY - 1991

    Y1 - 1991

    N2 - 1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology.

    AB - 1. The whole-cell configuration of the patch-clamp recording technique was used to characterize the electrophysiological properties of CRI-G1 insulin-secreting cells. 2. Current-clamp recordings demonstrated the excitable nature of these cells. 3. Voltage-clamp recordings revealed the presence of an inward Na+ current, an inward Ca2+ current and a delayed outward K+ conductance. 4. The electrophysiological properties of CRI-G1 closely resemble those of pancreatic beta-cells, thereby rendering this cell-line as a useful alternative to freshly isolated cells for the study of pancreatic beta-cell electrophysiology and pharmacology.

    KW - Rats

    KW - Animals

    KW - Membrane Potentials

    KW - Action Potentials

    KW - Islets of Langerhans

    KW - Models, Biological

    KW - Insulin

    KW - Cell Line

    U2 - 10.1016/0300-9629(91)90379-Q

    DO - 10.1016/0300-9629(91)90379-Q

    M3 - Article

    VL - 100

    SP - 613

    EP - 621

    JO - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology

    JF - Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology

    SN - 1095-6433

    IS - 3

    ER -