Widefield light sheet microscopy using an Airy beam combined with deep-learning super-resolution

Stella Corsetti (Lead / Corresponding author), Philip Wijesinghe, Persephone B. Poulton, Shuzo Sakata, Kushi Vyas, Simon Herrington, Jonathan Nylk, Federico M. Gasparoli, Kishan Dholakia (Lead / Corresponding author)

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Abstract

Imaging across length scales and in depth has been an important pursuit of widefield optical imaging. This promises to reveal fine cellular detail within a widefield snapshot of a tissue sample. Current advances often sacrifice resolution through selective sub-sampling to provide a wide field of view in a reasonable time scale. We demonstrate a new avenue for recovering high-resolution images from sub-sampled data in light sheet microscopy using deep-learning super-resolution. We combine this with the use of a widefield Airy beam to achieve high-resolution imaging over extended fields of view and depths. We characterise our method on fluorescent beads as test targets. We then demonstrate improvements in imaging amyloid plaques in a cleared brain from a mouse model of Alzheimer’s disease, and in excised healthy and cancerous colon and breast tissues. This development can be widely applied in all forms of light sheet microscopy to provide a two-fold increase in the dynamic range of the imaged length scale. It has the potential to provide further insight into neuroscience, developmental biology, and histopathology.
Original languageEnglish
Pages (from-to)1068-1083
Number of pages16
JournalOSA Continuum
Volume3
Issue number4
Early online date14 Apr 2020
DOIs
Publication statusPublished - 15 Apr 2020

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