Xenopus Ran-binding protein 1

Molecular interactions and effects on nuclear assembly in Xenopus egg extracts

Francisco J. Nicolas, Chuanmao Zhang, Mike Hughes, Martin W. Goldberg, Sandra J. Watton, Paul R. Clarke (Lead / Corresponding author)

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Ran is a nuclear GTPase implicated in nucleocytoplasmic transport, the maintenance of nuclear structure, mRNA processing, and cell cycle regulation. By two-hybrid interaction in yeast, we have identified a Xenopus homologue of Ran-binding protein 1 (RanBP1). Xenopus RanBP1 interacts specifically with the GTP-bound form of Ran and forms complexes in Xenopus egg extracts with Ran, importin-β/karyopherin-β and importin-α/karyopherin-α, but not p10, p120/RanBP7, RanBP2 or other nucleoporins. These complexes may play roles in the recycling of Ran and importins/karyopherins during nucleocytoplasmic transport. Increased concentrations of RanBP1 stabilise an interaction between Ran and RCC1 in egg extracts, inhibiting the exchange activity of RCC1 towards Ran. Under these conditions, the assembly of nuclei from chromatin is dramatically affected: the nuclei do not assemble a lamina and become very small with homogeneously condensed chromatin. They fail to actively import proteins and do not undergo DNA replication. By field emission in-lens scanning electron microscopy, we show that these nuclei have an intact nuclear envelope containing pore complexes, but the envelope is highly convoluted. However, RanBP1 does not directly inhibit nuclear protein import in assembled nuclei. These results suggest that RCC1 and/or Ran have a function early in nuclear assembly that is disrupted by RanBP1.

Original languageEnglish
Pages (from-to)3019-3030
Number of pages12
JournalJournal of Cell Science
Volume110
Issue number24
Publication statusPublished - 1 Dec 1997

Fingerprint

Karyopherins
Xenopus
Ovum
Cell Nucleus Active Transport
Nuclear Pore Complex Proteins
Chromatin Assembly and Disassembly
GTP Phosphohydrolases
Nuclear Envelope
Recycling
Nuclear Proteins
Guanosine Triphosphate
DNA Replication
Electron Scanning Microscopy
Lenses
Chromatin
ran-binding protein 1
Cell Cycle
Yeasts
Maintenance
Messenger RNA

Keywords

  • Nuclear structure
  • Ran
  • RanBP1
  • RCC1
  • Xenopus laevis

Cite this

Nicolas, F. J., Zhang, C., Hughes, M., Goldberg, M. W., Watton, S. J., & Clarke, P. R. (1997). Xenopus Ran-binding protein 1: Molecular interactions and effects on nuclear assembly in Xenopus egg extracts. Journal of Cell Science, 110(24), 3019-3030.
Nicolas, Francisco J. ; Zhang, Chuanmao ; Hughes, Mike ; Goldberg, Martin W. ; Watton, Sandra J. ; Clarke, Paul R. / Xenopus Ran-binding protein 1 : Molecular interactions and effects on nuclear assembly in Xenopus egg extracts. In: Journal of Cell Science. 1997 ; Vol. 110, No. 24. pp. 3019-3030.
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Nicolas, FJ, Zhang, C, Hughes, M, Goldberg, MW, Watton, SJ & Clarke, PR 1997, 'Xenopus Ran-binding protein 1: Molecular interactions and effects on nuclear assembly in Xenopus egg extracts', Journal of Cell Science, vol. 110, no. 24, pp. 3019-3030.

Xenopus Ran-binding protein 1 : Molecular interactions and effects on nuclear assembly in Xenopus egg extracts. / Nicolas, Francisco J.; Zhang, Chuanmao; Hughes, Mike; Goldberg, Martin W.; Watton, Sandra J.; Clarke, Paul R. (Lead / Corresponding author).

In: Journal of Cell Science, Vol. 110, No. 24, 01.12.1997, p. 3019-3030.

Research output: Contribution to journalArticle

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