Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum

Hazel McLellan, Miles R. Armstrong, Paul R. J. Birch (Lead / Corresponding author)

Research output: Chapter in Book/Report/Conference proceedingChapter (peer-reviewed)peer-review

Abstract

Yeast two-hybrid (Y2H) is a technique used to identify protein-protein interactions. It relies on interacting proteins bringing the two domains of a split transcription factor into close proximity, thereby reconstituting its ability to activate reporter genes. There are many variations on this technique. Here we provide an adapted protocol based on the Invitrogen ProQuest™ Two-Hybrid system, which has been used successfully to screen over 60 Phytophthora infestans pathogen effector proteins to identify candidate-interacting proteins in Solanum tuberosum, and has been used to identify many potato-potato protein-protein interactions.

Original languageEnglish
Title of host publicationSolanum tuberosum
Subtitle of host publicationMethods and Protocols
EditorsDavid Dobnik, Kristina Gruden, Živa Ramšak, Anna Coll
Place of PublicationNew York
PublisherHumana Press
Pages95-110
Number of pages16
Edition1
ISBN (Electronic)9781071616093
ISBN (Print)9781071616086 (hbk), 9781071616116 (pbk)
DOIs
Publication statusPublished - 2021

Publication series

NameMethods in Molecular Biology
Volume2354
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Phytophthora infestans
  • Potato
  • Protein-protein interactions
  • Yeast two-hybrid

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