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Yeast two-hybrid (Y2H) is a technique used to identify protein-protein interactions. It relies on interacting proteins bringing the two domains of a split transcription factor into close proximity, thereby reconstituting its ability to activate reporter genes. There are many variations on this technique. Here we provide an adapted protocol based on the Invitrogen ProQuest™ Two-Hybrid system, which has been used successfully to screen over 60 Phytophthora infestans pathogen effector proteins to identify candidate-interacting proteins in Solanum tuberosum, and has been used to identify many potato-potato protein-protein interactions.
|Title of host publication||Solanum tuberosum|
|Subtitle of host publication||Methods and Protocols|
|Editors||David Dobnik, Kristina Gruden, Živa Ramšak, Anna Coll|
|Place of Publication||New York|
|Number of pages||16|
|ISBN (Print)||9781071616086 (hbk), 9781071616116 (pbk)|
|Publication status||Published - 2021|
|Name||Methods in Molecular Biology|
- Phytophthora infestans
- Protein-protein interactions
- Yeast two-hybrid
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