Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum

Hazel McLellan; Miles R. Armstrong; Paul R. J. Birch

doi:10.1007/978-1-0716-1609-3_4

Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum

Hazel McLellan, Miles R. Armstrong, Paul R. J. Birch (Lead / Corresponding author)

Plant Sciences

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

3 Citations (Scopus)

Abstract

Yeast two-hybrid (Y2H) is a technique used to identify protein-protein interactions. It relies on interacting proteins bringing the two domains of a split transcription factor into close proximity, thereby reconstituting its ability to activate reporter genes. There are many variations on this technique. Here we provide an adapted protocol based on the Invitrogen ProQuest™ Two-Hybrid system, which has been used successfully to screen over 60 Phytophthora infestans pathogen effector proteins to identify candidate-interacting proteins in Solanum tuberosum, and has been used to identify many potato-potato protein-protein interactions.

Original language	English
Title of host publication	Solanum tuberosum
Subtitle of host publication	Methods and Protocols
Editors	David Dobnik, Kristina Gruden, Živa Ramšak, Anna Coll
Place of Publication	New York
Publisher	Humana Press
Pages	95-110
Number of pages	16
Edition	1
ISBN (Electronic)	9781071616093
ISBN (Print)	9781071616086 (hbk), 9781071616116 (pbk)
DOIs	https://doi.org/10.1007/978-1-0716-1609-3_4
Publication status	Published - 2021

Publication series

Name	Methods in Molecular Biology
Volume	2354
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Phytophthora infestans
Potato
Protein-protein interactions
Yeast two-hybrid

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-0716-1609-3_4

Cite this

McLellan, H., Armstrong, M. R., & Birch, P. R. J. (2021). Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum. In D. Dobnik, K. Gruden, Ž. Ramšak, & A. Coll (Eds.), Solanum tuberosum: Methods and Protocols (1 ed., pp. 95-110). (Methods in Molecular Biology; Vol. 2354). Humana Press. https://doi.org/10.1007/978-1-0716-1609-3_4

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abstract = "Yeast two-hybrid (Y2H) is a technique used to identify protein-protein interactions. It relies on interacting proteins bringing the two domains of a split transcription factor into close proximity, thereby reconstituting its ability to activate reporter genes. There are many variations on this technique. Here we provide an adapted protocol based on the Invitrogen ProQuest{\texttrademark} Two-Hybrid system, which has been used successfully to screen over 60 Phytophthora infestans pathogen effector proteins to identify candidate-interacting proteins in Solanum tuberosum, and has been used to identify many potato-potato protein-protein interactions.",

keywords = "Phytophthora infestans, Potato, Protein-protein interactions, Yeast two-hybrid",

author = "Hazel McLellan and Armstrong, {Miles R.} and Birch, {Paul R. J.}",

note = "This work was supported by BBSRC grants BB/P020569/1, BB/N009967/1, BB/K018183/1, and BB/G015244/1 to P.R.J.B. {\textcopyright} 2021. Springer Science+Business Media, LLC, part of Springer Nature.",

year = "2021",

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language = "English",

isbn = "9781071616086 (hbk)",

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publisher = "Humana Press",

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Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum. / McLellan, Hazel; Armstrong, Miles R.; Birch, Paul R. J. (Lead / Corresponding author).
Solanum tuberosum: Methods and Protocols. ed. / David Dobnik; Kristina Gruden; Živa Ramšak; Anna Coll. 1. ed. New York: Humana Press, 2021. p. 95-110 (Methods in Molecular Biology; Vol. 2354).

Research output: Chapter in Book/Report/Conference proceeding › Chapter (peer-reviewed) › peer-review

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T1 - Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum

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AU - Armstrong, Miles R.

AU - Birch, Paul R. J.

PY - 2021

Y1 - 2021

N2 - Yeast two-hybrid (Y2H) is a technique used to identify protein-protein interactions. It relies on interacting proteins bringing the two domains of a split transcription factor into close proximity, thereby reconstituting its ability to activate reporter genes. There are many variations on this technique. Here we provide an adapted protocol based on the Invitrogen ProQuest™ Two-Hybrid system, which has been used successfully to screen over 60 Phytophthora infestans pathogen effector proteins to identify candidate-interacting proteins in Solanum tuberosum, and has been used to identify many potato-potato protein-protein interactions.

AB - Yeast two-hybrid (Y2H) is a technique used to identify protein-protein interactions. It relies on interacting proteins bringing the two domains of a split transcription factor into close proximity, thereby reconstituting its ability to activate reporter genes. There are many variations on this technique. Here we provide an adapted protocol based on the Invitrogen ProQuest™ Two-Hybrid system, which has been used successfully to screen over 60 Phytophthora infestans pathogen effector proteins to identify candidate-interacting proteins in Solanum tuberosum, and has been used to identify many potato-potato protein-protein interactions.

KW - Phytophthora infestans

KW - Potato

KW - Protein-protein interactions

KW - Yeast two-hybrid

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U2 - 10.1007/978-1-0716-1609-3_4

DO - 10.1007/978-1-0716-1609-3_4

M3 - Chapter (peer-reviewed)

C2 - 34448156

SN - 9781071616086 (hbk)

SN - 9781071616116 (pbk)

T3 - Methods in Molecular Biology

SP - 95

EP - 110

BT - Solanum tuberosum

A2 - Dobnik, David

A2 - Gruden, Kristina

A2 - Ramšak, Živa

A2 - Coll, Anna

PB - Humana Press

CY - New York

ER -

Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

New Approaches to Undermine Late Blight Disease by Exploiting an Understanding of Ubiquitin E3 Ligases that Positively Regulate Immunity

Undermining Effector-Targeted Susceptibility Factors to Provide Late Blight Resistance (Industrial Partnership award)

The Contribution of Phytophthora Effectors to Host Range and Non-Host Resistance (Joint with James Hutton Institute and University of Warwick and Industrial Partners)

Cite this

Yeast Two-Hybrid Screening for Identification of Protein-Protein Interactions in Solanum tuberosum

Abstract

Publication series

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Projects

New Approaches to Undermine Late Blight Disease by Exploiting an Understanding of Ubiquitin E3 Ligases that Positively Regulate Immunity

Undermining Effector-Targeted Susceptibility Factors to Provide Late Blight Resistance (Industrial Partnership award)

The Contribution of Phytophthora Effectors to Host Range and Non-Host Resistance (Joint with James Hutton Institute and University of Warwick and Industrial Partners)

Cite this