YelA, a putative Dictyostelium translational regulator, acts as antagonist of DIF-1 signaling to control cell-type proportioning

Yoko Yamada (Lead / Corresponding author), Chris Sugden, Jeffrey G. Williams

    Research output: Contribution to journalArticlepeer-review

    184 Downloads (Pure)

    Abstract

    DIF-1 (differentiation-inducing factor1) is a polyketide produced by Dictyostelium prespore cells which induces initially uncommitted cells to differentiate as prestalk cells. Exposure of cells to DIF-1 causes transitory hypo-phosphorylation of seven serine residues in YelA, a protein with a region of strong homology to the MIF4G domain of the eukaryotic initiation factor eIF4G. Based upon its domain architecture, which in one important aspect closely resembles that of Death-Associated Protein 5 (DAP5), we predict a role in stimulating internal ribosome entry-driven mRNA translation. The two paradigmatic DIF-1 inducible genes are ecmA (extracellular matrixA) and ecmB. In support of a YelA function in DIF-1 signaling, a YelA null strain showed greatly increased expression of ecmA and ecmB in response to DIF-1. Also, during normal development in the null strain, expression of the two genes is accelerated. This is particularly evident for ecmB, a marker of stalk tube and supporting structure differentiation. Mutants in DIF-1 bio-synthesis or signaling display a rudimentary or no basal disc and, conversely, YelA null mutants produce fruiting bodies with a highly enlarged basal disc that ectopically expresses a stalk tube-specific marker. Thus YelA acts as an antagonist of DIF-1 signaling, with a consequent effect on cell type proportioning and it is predicted to act as a translational regulator.

    Original languageEnglish
    Pages (from-to)35-42
    Number of pages8
    JournalInternational Journal of Developmental Biology
    Volume61
    Issue number1-2
    DOIs
    Publication statusPublished - 2017

    Keywords

    • Journal article
    • Dictyostelium
    • DIF-1
    • MIF4G domain
    • Translational regulation
    • DAP5

    Fingerprint

    Dive into the research topics of 'YelA, a putative Dictyostelium translational regulator, acts as antagonist of DIF-1 signaling to control cell-type proportioning'. Together they form a unique fingerprint.

    Cite this