Ypt1/Rab1 regulates Hrr25/CK1δ kinase activity in ER-Golgi traffic and macroautophagy.

Juan Wang, Saralin Davis, Shekar Menon, Jinzhong Zhang, Jingzhen Ding, Serena Cervantes, Elizabeth Miller, Yu Jiang, Susan Ferro-Novick (Lead / Corresponding author)

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58 Citations (Scopus)
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ER-derived COPII-coated vesicles are conventionally targeted to the Golgi. However, during cell stress these vesicles also become a membrane source for autophagosomes, distinct organelles that target cellular components for degradation. How the itinerary of COPII vesicles is coordinated on these pathways remains unknown. Phosphorylation of the COPII coat by casein kinase 1 (CK1), Hrr25, contributes to the directional delivery of ER-derived vesicles to the Golgi. CK1 family members are thought to be constitutively active kinases that are regulated through their subcellular localization. Instead, we show here that the Rab GTPase Ypt1/Rab1 binds and activates Hrr25/CK1δ to spatially regulate its kinase activity. Consistent with a role for COPII vesicles and Hrr25 in membrane traffic and autophagosome biogenesis, hrr25 mutants were defective in ER–Golgi traffic and macroautophagy. These studies are likely to serve as a paradigm for how CK1 kinases act in membrane traffic.
Original languageEnglish
Pages (from-to)273–285
Number of pages13
JournalJournal of Cell Biology
Issue number2
Publication statusPublished - 20 Jul 2015


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