YuaB Functions Synergistically with the Exopolysaccharide and TasA Amyloid Fibers To Allow Biofilm Formation by Bacillus subtilis

Adam Ostrowski, Angela Mehert, Alan Prescott, Taryn B. Kiley, Nicola R. Stanley-Wall

    Research output: Contribution to journalArticle

    67 Citations (Scopus)

    Abstract

    During biofilm formation by Bacillus subtilis, two extracellular matrix components are synthesized, namely, the TasA amyloid fibers and an exopolysaccharide. In addition, a small protein called YuaB has been shown to allow the biofilm to form. The regulatory protein DegU is known to initiate biofilm formation. In this report we show that the main role of DegU during biofilm formation is to indirectly drive the activation of transcription from the yuaB promoter. The N terminus of YuaB constitutes a signal peptide for the Sec transport system. Here we show that the presence of the signal peptide is required for YuaB function. In addition we demonstrate that upon export of YuaB from the cytoplasm, it localizes to the cell wall. We continue with evidence that increased production of TasA and the exopolysaccharide is not sufficient to overcome the effects of a mutation in yuaB, demonstrating the unique involvement of YuaB in forming a biofilm. In line with this, YuaB is not involved in correct synthesis, export, or polymerization of either the TasA amyloid fibers or the exopolysaccharide. Taken together, these findings identify YuaB as a protein that plays a novel role during biofilm formation. We hypothesize that YuaB functions synergistically with the known components of the biofilm matrix to facilitate the assembly of the biofilm matrix.

    Original languageEnglish
    Pages (from-to)4821-4831
    Number of pages11
    JournalJournal of Bacteriology
    Volume193
    Issue number18
    DOIs
    Publication statusPublished - Sep 2011

    Keywords

    • Bacillus subtilis
    • Bacterial Proteins
    • Biofilms
    • Cell Wall
    • Polysaccharides, Bacterial
    • Protein Sorting Signals

    Cite this

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    title = "YuaB Functions Synergistically with the Exopolysaccharide and TasA Amyloid Fibers To Allow Biofilm Formation by Bacillus subtilis",
    abstract = "During biofilm formation by Bacillus subtilis, two extracellular matrix components are synthesized, namely, the TasA amyloid fibers and an exopolysaccharide. In addition, a small protein called YuaB has been shown to allow the biofilm to form. The regulatory protein DegU is known to initiate biofilm formation. In this report we show that the main role of DegU during biofilm formation is to indirectly drive the activation of transcription from the yuaB promoter. The N terminus of YuaB constitutes a signal peptide for the Sec transport system. Here we show that the presence of the signal peptide is required for YuaB function. In addition we demonstrate that upon export of YuaB from the cytoplasm, it localizes to the cell wall. We continue with evidence that increased production of TasA and the exopolysaccharide is not sufficient to overcome the effects of a mutation in yuaB, demonstrating the unique involvement of YuaB in forming a biofilm. In line with this, YuaB is not involved in correct synthesis, export, or polymerization of either the TasA amyloid fibers or the exopolysaccharide. Taken together, these findings identify YuaB as a protein that plays a novel role during biofilm formation. We hypothesize that YuaB functions synergistically with the known components of the biofilm matrix to facilitate the assembly of the biofilm matrix.",
    keywords = "Bacillus subtilis, Bacterial Proteins, Biofilms, Cell Wall, Polysaccharides, Bacterial, Protein Sorting Signals",
    author = "Adam Ostrowski and Angela Mehert and Alan Prescott and Kiley, {Taryn B.} and Stanley-Wall, {Nicola R.}",
    note = "Copyright {\circledC} 2011, American Society for Microbiology. All Rights Reserved.",
    year = "2011",
    month = "9",
    doi = "10.1128/JB.00223-11",
    language = "English",
    volume = "193",
    pages = "4821--4831",
    journal = "Journal of Bacteriology",
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    YuaB Functions Synergistically with the Exopolysaccharide and TasA Amyloid Fibers To Allow Biofilm Formation by Bacillus subtilis. / Ostrowski, Adam; Mehert, Angela; Prescott, Alan; Kiley, Taryn B.; Stanley-Wall, Nicola R.

    In: Journal of Bacteriology, Vol. 193, No. 18, 09.2011, p. 4821-4831.

    Research output: Contribution to journalArticle

    TY - JOUR

    T1 - YuaB Functions Synergistically with the Exopolysaccharide and TasA Amyloid Fibers To Allow Biofilm Formation by Bacillus subtilis

    AU - Ostrowski, Adam

    AU - Mehert, Angela

    AU - Prescott, Alan

    AU - Kiley, Taryn B.

    AU - Stanley-Wall, Nicola R.

    N1 - Copyright © 2011, American Society for Microbiology. All Rights Reserved.

    PY - 2011/9

    Y1 - 2011/9

    N2 - During biofilm formation by Bacillus subtilis, two extracellular matrix components are synthesized, namely, the TasA amyloid fibers and an exopolysaccharide. In addition, a small protein called YuaB has been shown to allow the biofilm to form. The regulatory protein DegU is known to initiate biofilm formation. In this report we show that the main role of DegU during biofilm formation is to indirectly drive the activation of transcription from the yuaB promoter. The N terminus of YuaB constitutes a signal peptide for the Sec transport system. Here we show that the presence of the signal peptide is required for YuaB function. In addition we demonstrate that upon export of YuaB from the cytoplasm, it localizes to the cell wall. We continue with evidence that increased production of TasA and the exopolysaccharide is not sufficient to overcome the effects of a mutation in yuaB, demonstrating the unique involvement of YuaB in forming a biofilm. In line with this, YuaB is not involved in correct synthesis, export, or polymerization of either the TasA amyloid fibers or the exopolysaccharide. Taken together, these findings identify YuaB as a protein that plays a novel role during biofilm formation. We hypothesize that YuaB functions synergistically with the known components of the biofilm matrix to facilitate the assembly of the biofilm matrix.

    AB - During biofilm formation by Bacillus subtilis, two extracellular matrix components are synthesized, namely, the TasA amyloid fibers and an exopolysaccharide. In addition, a small protein called YuaB has been shown to allow the biofilm to form. The regulatory protein DegU is known to initiate biofilm formation. In this report we show that the main role of DegU during biofilm formation is to indirectly drive the activation of transcription from the yuaB promoter. The N terminus of YuaB constitutes a signal peptide for the Sec transport system. Here we show that the presence of the signal peptide is required for YuaB function. In addition we demonstrate that upon export of YuaB from the cytoplasm, it localizes to the cell wall. We continue with evidence that increased production of TasA and the exopolysaccharide is not sufficient to overcome the effects of a mutation in yuaB, demonstrating the unique involvement of YuaB in forming a biofilm. In line with this, YuaB is not involved in correct synthesis, export, or polymerization of either the TasA amyloid fibers or the exopolysaccharide. Taken together, these findings identify YuaB as a protein that plays a novel role during biofilm formation. We hypothesize that YuaB functions synergistically with the known components of the biofilm matrix to facilitate the assembly of the biofilm matrix.

    KW - Bacillus subtilis

    KW - Bacterial Proteins

    KW - Biofilms

    KW - Cell Wall

    KW - Polysaccharides, Bacterial

    KW - Protein Sorting Signals

    U2 - 10.1128/JB.00223-11

    DO - 10.1128/JB.00223-11

    M3 - Article

    C2 - 21742882

    VL - 193

    SP - 4821

    EP - 4831

    JO - Journal of Bacteriology

    JF - Journal of Bacteriology

    SN - 0021-9193

    IS - 18

    ER -