Zooming in on Chromosome Dynamics

John K. Eykelenboom (Lead / Corresponding author), Tomoyuki U. Tanaka

Research output: Contribution to journalArticle

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Abstract

Until recently, our understanding of chromosome organisation in higher eukaryotic cells has been based on analyses of large-scale, low resolution changes in chromosomes structure. More recently, CRISPR-Cas9 technologies have allowed us to “zoom in” and visualise specific chromosome regions in live cells so that we can begin to examine in detail the dynamics of chromosome organisation in individual cells. In this review we discuss traditional methods of chromosome locus visualisation and look at how CRISPRCas9 gene-targeting methodologies have helped improve their application. We also describe recent developments of the CRISPR-Cas9 technology that enable visualisation of specific chromosome regions without the requirement for complex genetic manipulation.
Original languageEnglish
Pages (from-to)1422-1432
Number of pages11
JournalCell Cycle
Volume19
Issue number12
Early online date13 May 2020
DOIs
Publication statusPublished - 17 Jun 2020

Keywords

  • Chromosome structure
  • chromosome dynamics
  • live-cell imaging
  • fluorescent visualisation
  • CRISPR-Cas9 technology
  • fluorescent visualization

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