Projects per year
Abstract
Until recently, our understanding of chromosome organisation in higher eukaryotic cells has been based on analyses of large-scale, low resolution changes in chromosomes structure. More recently, CRISPR-Cas9 technologies have allowed us to “zoom in” and visualise specific chromosome regions in live cells so that we can begin to examine in detail the dynamics of chromosome organisation in individual cells. In this review we discuss traditional methods of chromosome locus visualisation and look at how CRISPRCas9 gene-targeting methodologies have helped improve their application. We also describe recent developments of the CRISPR-Cas9 technology that enable visualisation of specific chromosome regions without the requirement for complex genetic manipulation.
Original language | English |
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Pages (from-to) | 1422-1432 |
Number of pages | 11 |
Journal | Cell Cycle |
Volume | 19 |
Issue number | 12 |
Early online date | 13 May 2020 |
DOIs | |
Publication status | Published - 17 Jun 2020 |
Keywords
- Chromosome structure
- chromosome dynamics
- live-cell imaging
- fluorescent visualisation
- CRISPR-Cas9 technology
- fluorescent visualization
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology
- Developmental Biology
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Dive into the research topics of 'Zooming in on Chromosome Dynamics'. Together they form a unique fingerprint.Projects
- 3 Finished
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Molecular Mechanisms for the Final Step of Dissolving Sister Chromatid Cohesion at Anaphase Onset
Tanaka, T. (Investigator)
Biotechnology and Biological Sciences Research Council
1/10/19 → 30/09/23
Project: Research
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SELFCC: Chromosome Self-clearing Completes Sister Chromatid Separation
Tanaka, T. (Investigator)
COMMISSION OF THE EUROPEAN COMMUNITIES
1/04/13 → 30/09/18
Project: Research
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Molecular Mechanisms Regulating the Kinetochore-Microtubule Interaction in Mitosis (Principal Research Fellowship)
Tanaka, T. (Investigator)
1/04/12 → 30/04/21
Project: Research